Research

Sub-project 1: Exploration and reconstitution of intracellular enviornments

1.In situ structural studies of translational control and nonmembrane organelles

Laboratory for Translation Structural Biology
Team leader: Takuhiro Ito

We utilize cryo-electron tomography to investigate translational control and architectures of nonmembrane organelles cell-type and intercellular-position specifically.

2.Isolation of cellular environments and survey for the substrates

RNA Systems Biochemistry laboratory
Team leader: Shintaro Iwasaki

We aim to isolate the cellular environments through biochemical methods and also identify the protein clients protected by Hero proteins.

3.Single-molecule measurements in cellular environment

Cellular Informatics laboratory
Team leader: Yasushi Sako

We develop novel microscopic techniques to address the biophysical features maintained by cellular environments and also the that of biomolecules in the cellular environment.

Dissection of intracellular environmentsusing chromosome reconstitution assays in vitro

4.Dissection of intracellular environments using chromosome reconstitution assays in vitro

Chromosome Dynamics Laboratory
Team leader: Tatsuya Hirano

We use in vitro reconstitution assays to investigate the intracellular environments that enable proper assembly of large-scale chromosome structures.

5.Deciphering cellular dark matter for light response in plants

Synthetic Genomics Research Group
Team leader: Minami Matsui

We explore transcriptome of plant-genes by monochromatic lights and examine metabolome of plant-specific cell divisions.

Sub-project 2: Visualization and characterization of intracellular environments

Biomolecules in cellular environments

1.Structure and dynamics of biomolecules in cellular environments

Laboratory for Cellular Structural Biology
Team leader: Takanori Kigawa

We develop a new multi-scale simulation technique to investigate relations between sWe elucidate biomolecular structural dynamics in the cellular environment by using NMR spectroscopy and information science methodologies.

Structural biology utilizing the Hero proteins

2.Structural biology utilizing the Hero proteins

Laboratory for Protein Functional and Structural Biology
Team leader: Mikako Shirouzu

We investigate the effects of the Hero proteins on the protein complex preparation and structure determinations using cryo-electron microscopy.

Multi-scale simulations of protein structures and interactions in the cell

3.Multi-scale simulations of protein structures and interactions in the cell

Theoretical molecular science laboratory
Team leader: Yuji Sugita

We study disordered protein structures and interactions with other macromolecules in crowded cellular environments using multi-scale molecular dynamics simulations

Structural analyses of chromatin transcription and environmental factors

4.Structural analyses of chromatin transcription and environmental factors

Laboratory for Transcription Structural Biology
Team leader: Shun-ichi Sekine

We perform cryoEM analyses of transcription complexes to reveal the roles of intrinsically disordered domains or noncoding RNAs in chromatin transcription

NMR spectroscopy of structures and aggregations of intrinsically disordered proteins

5.NMR spectroscopy of structures and aggregations of intrinsically disordered proteins

RIKEN Center for Brain Science, Laboratory for Protein Conformation Diseases
Team Leader: Motomasa Tanaka

We develop a new in vitro reconstitution and cell model systems and investigate protein dynamics during amyloid formation and disaggregation by various biophysical methods including NMR spectroscopy.

Sub-project 1
1.In situ structural studies of translational control and nonmembrane organelles
Laboratory for Translation Structural Biology
Team leader: Takuhiro Ito
We utilize cryo-electron tomography to investigate translational control and architectures of nonmembrane organelles cell-type and intercellular-position specifically.
Sub-project 1
2.Isolation of cellular environments and survey for the substrates
RNA Systems Biochemistry laboratory
Team leader: Shintaro Iwasaki
We aim to isolate the cellular environments through biochemical methods and also identify the protein clients protected by Hero proteins.
Sub-project 1
3.Single-molecule measurements in cellular environment
Cellular Informatics laboratory
Team leader: Yasushi Sako
We develop novel microscopic techniques to address the biophysical features maintained by cellular environments and also the that of biomolecules in the cellular environment.
Sub-project 1
4.Dissection of intracellular environments using chromosome reconstitution assays in vitro
Chromosome Dynamics Laboratory
Team leader: Tatsuya Hirano
We use in vitro reconstitution assays to investigate the intracellular environments that enable proper assembly of large-scale chromosome structures.
Sub-project 1
5.Development of a new simulation technique for protein structure modeling
Theoretical molecular science laboratory
Team leader: Yuji Sugita
We develop a new multi-scale simulation technique to investigate relations between structure, function, and dynamics of biomolecules in the cellular environment.
Sub-project 2
1.Structure and dynamics of biomolecules in cellular environments
Laboratory for Cellular Structural Biology
Team leader: Takanori Kigawa
We elucidate biomolecular structural dynamics in the cellular environment by using NMR spectroscopy and information science methodologies.
Sub-project 2
2.Structural biology utilizing the Hero proteins
Laboratory for Protein Functional and Structural Biology
Team leader: Mikako Shirouzu
We investigate the effects of the Hero proteins on the protein complex preparation and structure determinations using cryo-electron microscopy.
Sub-project 2
3.Multi-scale simulations of protein structures and interactions in the cell
Theoretical molecular science laboratory
Team leader: Yuji Sugita
We study disordered protein structures and interactions with other macromolecules in crowded cellular environments using multi-scale molecular dynamics simulations
Sub-project 2
4.Structural analyses of chromatin transcription and environmental factors
Laboratory for Transcription Structural Biology
Team leader: Shun-ichi Sekine
We perform cryoEM analyses of transcription complexes to reveal the roles of intrinsically disordered domains or noncoding RNAs in chromatin transcription
Sub-project 2
5.NMR spectroscopy of structures and aggregations of intrinsically disordered proteins
RIKEN Center for Brain Science, Laboratory for Protein Conformation Diseases
Team Leader: Motomasa Tanaka
We develop a new in vitro reconstitution and cell model systems and investigate protein dynamics during amyloid formation and disaggregation by various biophysical methods including NMR spectroscopy.
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